全文获取类型
收费全文 | 29394篇 |
免费 | 2571篇 |
国内免费 | 2426篇 |
出版年
2024年 | 18篇 |
2023年 | 261篇 |
2022年 | 397篇 |
2021年 | 1326篇 |
2020年 | 1000篇 |
2019年 | 1268篇 |
2018年 | 1255篇 |
2017年 | 953篇 |
2016年 | 1307篇 |
2015年 | 1822篇 |
2014年 | 2162篇 |
2013年 | 2381篇 |
2012年 | 2678篇 |
2011年 | 2511篇 |
2010年 | 1639篇 |
2009年 | 1527篇 |
2008年 | 1690篇 |
2007年 | 1511篇 |
2006年 | 1283篇 |
2005年 | 1132篇 |
2004年 | 903篇 |
2003年 | 876篇 |
2002年 | 781篇 |
2001年 | 566篇 |
2000年 | 473篇 |
1999年 | 437篇 |
1998年 | 267篇 |
1997年 | 234篇 |
1996年 | 232篇 |
1995年 | 222篇 |
1994年 | 214篇 |
1993年 | 152篇 |
1992年 | 206篇 |
1991年 | 143篇 |
1990年 | 132篇 |
1989年 | 105篇 |
1988年 | 74篇 |
1987年 | 60篇 |
1986年 | 50篇 |
1985年 | 50篇 |
1984年 | 22篇 |
1983年 | 22篇 |
1982年 | 23篇 |
1981年 | 8篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1977年 | 2篇 |
1975年 | 3篇 |
1965年 | 1篇 |
1950年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
Yao Chen Jie Zhu Jun Mo Hongyu Yang Xueyang Jiang Hongzhi Lin 《Journal of enzyme inhibition and medicinal chemistry》2018,33(1):290-302
Small molecule cholinesterases inhibitor (ChEI) provides an effective therapeutic strategy to treat Alzheimer’s disease (AD). Currently, the discovery of new ChEI with multi-target effect is still of great importance. Herein, we report the synthesis, structure–activity relationship study and biological evaluation of a series of tacrine-cinnamic acid hybrids as new ChEIs. All target compounds are evaluated for their in vitro cholinesterase inhibitory activities. The representatives which show potent activity on cholinesterase, are evaluated for the amyloid β-protein self-aggregation inhibition and in vivo assays. The optimal compound 19, 27, and 30 (human AChE IC50?=?10.2?±?1.2, 16.5?±?1.7, and 15.3?±?1.8?nM, respectively) show good performance in ameliorating the scopolamine-induced cognition impairment and preliminary safety in hepatotoxicity evaluation. These compounds deserve further evaluation for the development of new therapeutic agents against AD. 相似文献
102.
103.
Xiaorui Cui Mingpeng Li Zhengchu He Lin Hu Jianping Liu Jianhui Yan Liming Hua 《Cell biochemistry and function》2020,38(8):1025-1035
104.
Y. Jiang 《Biochimica et Biophysica Acta (BBA)/General Subjects》1994,1201(1):76-84
Proteolysis of the hydroxylase component of soluble methane monooxygenase (MMO) with trypsin yielded a protein which retained 50% activity in a standard MMO assay. In an H2O2-driven assay, in which H2O2 replaced two of the protein components, NADH and O2 used in the standard assay, the proteolysed hydroxylase retained full activity for ethane, propane and propene, but had a 2–3 fold increase with methane as substrate. Several crosslinking reagents have been tested for their ability to stabilise the proteolysed form of the hydroxylase. Using polyoxyethylene bis(imidazolyl carbonyl) (Mr 3350) as the crosslinking agent, increased thermostability of the hydroxylase was observed. Activated methoxypolyethylene glycol (Mr 5000) was used to modify the hydroxylase which was now soluble in organic solvents as well as water and could be activated by H2O2. The glycol-modified hydroxylase functioned well in organic solvents in the catalysis of propene oxidation. 相似文献
105.
Comparative embryogenesis of Mecoptera and Lepidoptera with special reference to the abdominal prolegs 下载免费PDF全文
The eruciform larvae of holometabolous insects are primarily characterized by bearing a varying number of abdominal prolegs in addition to three pairs of thoracic legs. However, whether the prolegs are evolutionarily homologous among different insect orders is still a disputable issue. We examined the embryonic features and histological structure of the prolegs of the scorpionfly Panorpa byersi Hua and Huang (Mecoptera: Panorpidae) and the Oriental armyworm Mythimna separata (Walker) (Lepidoptera: Noctuidae) to investigate whether the prolegs are homologous between these two holometabolous insect orders. In the scorpionfly, paired lateral process primordia arise on abdominal segments I–VIII (A1–A8) in line with the thoracic legs in early embryonic stages, but degenerate into triangular protuberances in later stages, and paired medial processes appear along the midventral line before dorsal closure and eventually develop into unjointed, cone‐shaped prolegs. Histological observation showed that the lumina of the prolegs are not continuous with the hemocoel, differing distinctly from that of the basic appendicular plan of thoracic legs. These results suggest that the prolegs are likely secondary outgrowths in Mecoptera. In the armyworm, lateral process primordia appear on A1–A10 in alignment with the thoracic legs in the early embryonic stages, although only the rudiments on A3–A6 and A10 develop into segmented prolegs with the lumina continuous with the hemocoel and others degenerate eventually, suggesting that the prolegs are true segmental appendages serially homologous with the thoracic legs in Lepidoptera. Therefore, we conclude that the larval prolegs are likely not evolutionarily homologous between Mecoptera and Lepidoptera. J. Morphol. 277:585–593, 2016. © 2016 Wiley Periodicals, Inc. 相似文献
106.
107.
108.
109.
This paper describes a generic algorithm for finding restrictionsites within DNA sequences. The genericity ofthe algorithm is made possible through the use of set theory.Basic elements of DNA sequences, i.e. nucleotides (bases), arerepresented in sets, and DNA sequences, whether specific, ambiguousor even protein-coding, are represented as sequences of thosesets. The set intersection operation demonstrates its abilityto perform pattern-matching correctly on various DNA sequences.The performance analysis showed that the degree of complexityof the pattern matching is reduced from exponential to linear.An example is given to show the actual and potential restrictionsites, derived by the generic algorithm, in the DNA sequencetemplate coding for a synthetic calmodulin.
Received on October 2, 1990; accepted on December 18, 1990 相似文献
110.
A great variety of vertebrate cells contain detectable amounts of lectins, able to stimulate the initiation of cellular DNA synthesis. One of them, sarcolectin (SCL) can block interferon (IFN) action, by inhibiting the synthesis and the expression of the IFN dependent secondary proteins. As a result, the IFN-induced antiviral state is abolished in the cells, which likely facilitates their replication. We identified a major 65 kDa and a minor 55 kDa protein, which could carry these cellular functions. Their purification, especially that of the 65 kDa, was difficult, because of the proximity of albumin. We devised therefore a two-step primary separation, followed by a four-step final purification, which are reported here. The purification was controlled by high pressure liquid chromatography (HPLC), SDS-PAGE electrophoresis and identified by Western blots. We found that only the minor 55 kDa protein can be considered as being sarcolectin, while the major 65 kDa band results from the binding of some SCL molecules to albumin. The major biological functions, namely, stimulation of DNA synthesis and cell agglutination were preserved to the end of the last purification step. This work is requisite for establishing the molecular structure of SCL by recombinant DNA technology. 相似文献